Journal: IBRO Neuroscience Reports

Article Title: A key protein from Borrelia burgdorferi could stimulate cytokines in human microglial cells and inhibitory effects of Cucurbitacin IIa

doi: 10.1016/j.ibneur.2023.11.004

Figure Lengend Snippet: Gene primers in this study.

Article Snippet: ELISA was performed using Human IL-6 ELISA kit, Human IL-8 ELISA kit, and Human CXCL10 ELISA kit (Dakewe Bio-engineering Co., Ltd, Shenzhen, China) following the manufacturer’s instructions.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: A key protein from Borrelia burgdorferi could stimulate cytokines in human microglial cells and inhibitory effects of Cucurbitacin IIa

doi: 10.1016/j.ibneur.2023.11.004

Figure Lengend Snippet: Concentrations of 48 cytokines detected by cytokine liquid array (pg/mL).

Article Snippet: ELISA was performed using Human IL-6 ELISA kit, Human IL-8 ELISA kit, and Human CXCL10 ELISA kit (Dakewe Bio-engineering Co., Ltd, Shenzhen, China) following the manufacturer’s instructions.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: A key protein from Borrelia burgdorferi could stimulate cytokines in human microglial cells and inhibitory effects of Cucurbitacin IIa

doi: 10.1016/j.ibneur.2023.11.004

Figure Lengend Snippet: Comparison of IL-6, IL-8, CCL2, CCL5, CXCL1, and CXCL10 mRNA relative expression in different groups at the same time. (A) IL-6. (B) IL-8. (C) CCL2. (D) CCL5. (E) CXCL1. (F) CXCL10. (G) Drawing statement. Compared to the negative control group at the same time point, the relative expression of IL-6, IL-8, CCL2, CCL5, CXCL1, and CXCL10 mRNA increased significantly in both the LPS group and rBmpA group, while the mRNA expression of these factors was significantly lower in the combination of rBmpA and CuIIa group compared with the rBmpA alone group. Two-way ANOVA and Tukey’s multiple comparison test were used to evaluate the statistical significance between means and SEM of quadruplicate data sets, * P < 0.05, * * P < 0.01, * ** P < 0.001.

Article Snippet: ELISA was performed using Human IL-6 ELISA kit, Human IL-8 ELISA kit, and Human CXCL10 ELISA kit (Dakewe Bio-engineering Co., Ltd, Shenzhen, China) following the manufacturer’s instructions.

Techniques: Comparison, Expressing, Negative Control

Journal: IBRO Neuroscience Reports

Article Title: A key protein from Borrelia burgdorferi could stimulate cytokines in human microglial cells and inhibitory effects of Cucurbitacin IIa

doi: 10.1016/j.ibneur.2023.11.004

Figure Lengend Snippet: Comparison of IL-6, IL-8, CXCL10 concentration of cells in different groups of the same time. (A) IL-6. (B) IL-8. (C) CXCL10. (D) Drawing statement. Compared with the negative control group, the expression levels of IL-6, IL-8 and CXCL10 were significantly increased in LPS group and rBmpA group, while significantly decreased in rBmpA plus CuIIa group. Two-way ANOVA and Tukey’s multiple comparison test were used to evaluate the statistical significance between means and SEM of triplicate data sets, * P < 0.05, * * P < 0.01, * ** P < 0.001.

Article Snippet: ELISA was performed using Human IL-6 ELISA kit, Human IL-8 ELISA kit, and Human CXCL10 ELISA kit (Dakewe Bio-engineering Co., Ltd, Shenzhen, China) following the manufacturer’s instructions.

Techniques: Comparison, Concentration Assay, Negative Control, Expressing